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Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a h...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413556/ https://www.ncbi.nlm.nih.gov/pubmed/30319135 http://dx.doi.org/10.4103/aja.aja_80_18 |
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author | Kim, Bang-Jin Kim, Yong-Hee Oh, Myeong-Geun Kim, Ki-Jung Jung, Sang-Eun Jin, Ju-Hee Kim, Sun-Uk Min, Kwan-Sik Ryu, Buom-Yong |
author_facet | Kim, Bang-Jin Kim, Yong-Hee Oh, Myeong-Geun Kim, Ki-Jung Jung, Sang-Eun Jin, Ju-Hee Kim, Sun-Uk Min, Kwan-Sik Ryu, Buom-Yong |
author_sort | Kim, Bang-Jin |
collection | PubMed |
description | Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species. |
format | Online Article Text |
id | pubmed-6413556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64135562019-04-09 Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats Kim, Bang-Jin Kim, Yong-Hee Oh, Myeong-Geun Kim, Ki-Jung Jung, Sang-Eun Jin, Ju-Hee Kim, Sun-Uk Min, Kwan-Sik Ryu, Buom-Yong Asian J Androl Original Article Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species. Medknow Publications & Media Pvt Ltd 2019 2018-10-12 /pmc/articles/PMC6413556/ /pubmed/30319135 http://dx.doi.org/10.4103/aja.aja_80_18 Text en Copyright: © The Author(s)(2018) http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Kim, Bang-Jin Kim, Yong-Hee Oh, Myeong-Geun Kim, Ki-Jung Jung, Sang-Eun Jin, Ju-Hee Kim, Sun-Uk Min, Kwan-Sik Ryu, Buom-Yong Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title | Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title_full | Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title_fullStr | Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title_full_unstemmed | Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title_short | Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
title_sort | direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413556/ https://www.ncbi.nlm.nih.gov/pubmed/30319135 http://dx.doi.org/10.4103/aja.aja_80_18 |
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