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Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats

Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a h...

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Autores principales: Kim, Bang-Jin, Kim, Yong-Hee, Oh, Myeong-Geun, Kim, Ki-Jung, Jung, Sang-Eun, Jin, Ju-Hee, Kim, Sun-Uk, Min, Kwan-Sik, Ryu, Buom-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413556/
https://www.ncbi.nlm.nih.gov/pubmed/30319135
http://dx.doi.org/10.4103/aja.aja_80_18
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author Kim, Bang-Jin
Kim, Yong-Hee
Oh, Myeong-Geun
Kim, Ki-Jung
Jung, Sang-Eun
Jin, Ju-Hee
Kim, Sun-Uk
Min, Kwan-Sik
Ryu, Buom-Yong
author_facet Kim, Bang-Jin
Kim, Yong-Hee
Oh, Myeong-Geun
Kim, Ki-Jung
Jung, Sang-Eun
Jin, Ju-Hee
Kim, Sun-Uk
Min, Kwan-Sik
Ryu, Buom-Yong
author_sort Kim, Bang-Jin
collection PubMed
description Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species.
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spelling pubmed-64135562019-04-09 Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats Kim, Bang-Jin Kim, Yong-Hee Oh, Myeong-Geun Kim, Ki-Jung Jung, Sang-Eun Jin, Ju-Hee Kim, Sun-Uk Min, Kwan-Sik Ryu, Buom-Yong Asian J Androl Original Article Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species. Medknow Publications & Media Pvt Ltd 2019 2018-10-12 /pmc/articles/PMC6413556/ /pubmed/30319135 http://dx.doi.org/10.4103/aja.aja_80_18 Text en Copyright: © The Author(s)(2018) http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kim, Bang-Jin
Kim, Yong-Hee
Oh, Myeong-Geun
Kim, Ki-Jung
Jung, Sang-Eun
Jin, Ju-Hee
Kim, Sun-Uk
Min, Kwan-Sik
Ryu, Buom-Yong
Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title_full Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title_fullStr Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title_full_unstemmed Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title_short Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
title_sort direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413556/
https://www.ncbi.nlm.nih.gov/pubmed/30319135
http://dx.doi.org/10.4103/aja.aja_80_18
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