Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing

Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for targeted RNA-sequencing that uses complex pools of reverse transcription primers to enable sequencing enrichment at user-sele...

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Detalles Bibliográficos
Autores principales: Xu, Hansen, Fair, Benjamin J., Dwyer, Zachary W., Gildea, Michael, Pleiss, Jeffrey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414223/
https://www.ncbi.nlm.nih.gov/pubmed/30573814
http://dx.doi.org/10.1038/s41592-018-0258-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414223/
https://www.ncbi.nlm.nih.gov/pubmed/30573814
http://dx.doi.org/10.1038/s41592-018-0258-x

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