A simple genotyping method to detect small CRISPR-Cas9 induced indels by agarose gel electrophoresis

CRISPR gene editing creates indels in targeted genes that are detected by genotyping. Separating PCR products generated from wild-type versus mutant alleles with small indels based on size is beyond the resolution capacity of regular agarose gel electrophoresis. To overcome this limitation, we devel...

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Detalles Bibliográficos
Autores principales: Bhattacharya, Debanjan, Van Meir, Erwin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418129/
https://www.ncbi.nlm.nih.gov/pubmed/30872606
http://dx.doi.org/10.1038/s41598-019-39950-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418129/
https://www.ncbi.nlm.nih.gov/pubmed/30872606
http://dx.doi.org/10.1038/s41598-019-39950-4

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