Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes

BACKGROUND: The CRISPR/Cas9 is currently the predominant technology to enhance the genome editing efficiency in eukaryotes. Established tools for many fungal species exist while most of them are based on in vivo expressed Cas9 and guide RNA (gRNA). Alternatively, in vitro assembled Cas9 and gRNA rib...

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Detalles Bibliográficos
Autores principales: Kuivanen, Joosu, Korja, Veera, Holmström, Sami, Richard, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419801/
https://www.ncbi.nlm.nih.gov/pubmed/30923622
http://dx.doi.org/10.1186/s40694-019-0066-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419801/
https://www.ncbi.nlm.nih.gov/pubmed/30923622
http://dx.doi.org/10.1186/s40694-019-0066-9

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