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A universal method for sensitive and cell-free detection of CRISPR-associated nucleases

A multitude of biological applications for CRISPR-associated (Cas) nucleases have propelled the development of robust cell-based methods for quantitation of on- and off-target activities of these nucleases. However, emerging applications of these nucleases require cell-free methods that are simple,...

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Detalles Bibliográficos
Autores principales: Cox, Kurt J., Subramanian, Hari K. K., Samaniego, Christian Cuba, Franco, Elisa, Choudhary, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419926/
https://www.ncbi.nlm.nih.gov/pubmed/30996981
http://dx.doi.org/10.1039/c8sc03426e