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Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although tw...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420780/ https://www.ncbi.nlm.nih.gov/pubmed/30726740 http://dx.doi.org/10.1016/j.celrep.2019.01.052 |
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author | Salazar, Vanessa Jagger, Brett W. Mongkolsapaya, Juthathip Burgomaster, Katherine E. Dejnirattisai, Wanwisa Winkler, Emma S. Fernandez, Estefania Nelson, Christopher A. Fremont, Daved H. Pierson, Theodore C. Crowe, James E. Screaton, Gavin R. Diamond, Michael S. |
author_facet | Salazar, Vanessa Jagger, Brett W. Mongkolsapaya, Juthathip Burgomaster, Katherine E. Dejnirattisai, Wanwisa Winkler, Emma S. Fernandez, Estefania Nelson, Christopher A. Fremont, Daved H. Pierson, Theodore C. Crowe, James E. Screaton, Gavin R. Diamond, Michael S. |
author_sort | Salazar, Vanessa |
collection | PubMed |
description | Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although two SPOV strains failed to cause disease in immunocompetent mice, each accumulated in the brain, spleen, eye, testis, and kidney when type I interferon signaling was blocked and unexpectedly caused infection, immune cell infiltration, and swelling in the ankle. In pregnant mice, SPOV replicated in the placenta and fetus but did not cause placental insufficiency or microcephaly. We identified human antibodies from ZIKV or DENV immune subjects that neutralized SPOV infection and protected against lethal challenge. Our experiments describe similarities and differences in clinical syndromes between SPOV and ZIKV and suggest that their serological relatedness has implications for antibody therapeutics and flavivirus vaccine development. |
format | Online Article Text |
id | pubmed-6420780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-64207802019-03-16 Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice Salazar, Vanessa Jagger, Brett W. Mongkolsapaya, Juthathip Burgomaster, Katherine E. Dejnirattisai, Wanwisa Winkler, Emma S. Fernandez, Estefania Nelson, Christopher A. Fremont, Daved H. Pierson, Theodore C. Crowe, James E. Screaton, Gavin R. Diamond, Michael S. Cell Rep Article Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although two SPOV strains failed to cause disease in immunocompetent mice, each accumulated in the brain, spleen, eye, testis, and kidney when type I interferon signaling was blocked and unexpectedly caused infection, immune cell infiltration, and swelling in the ankle. In pregnant mice, SPOV replicated in the placenta and fetus but did not cause placental insufficiency or microcephaly. We identified human antibodies from ZIKV or DENV immune subjects that neutralized SPOV infection and protected against lethal challenge. Our experiments describe similarities and differences in clinical syndromes between SPOV and ZIKV and suggest that their serological relatedness has implications for antibody therapeutics and flavivirus vaccine development. 2019-02-05 /pmc/articles/PMC6420780/ /pubmed/30726740 http://dx.doi.org/10.1016/j.celrep.2019.01.052 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Salazar, Vanessa Jagger, Brett W. Mongkolsapaya, Juthathip Burgomaster, Katherine E. Dejnirattisai, Wanwisa Winkler, Emma S. Fernandez, Estefania Nelson, Christopher A. Fremont, Daved H. Pierson, Theodore C. Crowe, James E. Screaton, Gavin R. Diamond, Michael S. Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title | Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title_full | Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title_fullStr | Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title_full_unstemmed | Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title_short | Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice |
title_sort | dengue and zika virus cross-reactive human monoclonal antibodies protect against spondweni virus infection and pathogenesis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420780/ https://www.ncbi.nlm.nih.gov/pubmed/30726740 http://dx.doi.org/10.1016/j.celrep.2019.01.052 |
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