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Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice

Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although tw...

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Autores principales: Salazar, Vanessa, Jagger, Brett W., Mongkolsapaya, Juthathip, Burgomaster, Katherine E., Dejnirattisai, Wanwisa, Winkler, Emma S., Fernandez, Estefania, Nelson, Christopher A., Fremont, Daved H., Pierson, Theodore C., Crowe, James E., Screaton, Gavin R., Diamond, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420780/
https://www.ncbi.nlm.nih.gov/pubmed/30726740
http://dx.doi.org/10.1016/j.celrep.2019.01.052
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author Salazar, Vanessa
Jagger, Brett W.
Mongkolsapaya, Juthathip
Burgomaster, Katherine E.
Dejnirattisai, Wanwisa
Winkler, Emma S.
Fernandez, Estefania
Nelson, Christopher A.
Fremont, Daved H.
Pierson, Theodore C.
Crowe, James E.
Screaton, Gavin R.
Diamond, Michael S.
author_facet Salazar, Vanessa
Jagger, Brett W.
Mongkolsapaya, Juthathip
Burgomaster, Katherine E.
Dejnirattisai, Wanwisa
Winkler, Emma S.
Fernandez, Estefania
Nelson, Christopher A.
Fremont, Daved H.
Pierson, Theodore C.
Crowe, James E.
Screaton, Gavin R.
Diamond, Michael S.
author_sort Salazar, Vanessa
collection PubMed
description Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although two SPOV strains failed to cause disease in immunocompetent mice, each accumulated in the brain, spleen, eye, testis, and kidney when type I interferon signaling was blocked and unexpectedly caused infection, immune cell infiltration, and swelling in the ankle. In pregnant mice, SPOV replicated in the placenta and fetus but did not cause placental insufficiency or microcephaly. We identified human antibodies from ZIKV or DENV immune subjects that neutralized SPOV infection and protected against lethal challenge. Our experiments describe similarities and differences in clinical syndromes between SPOV and ZIKV and suggest that their serological relatedness has implications for antibody therapeutics and flavivirus vaccine development.
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spelling pubmed-64207802019-03-16 Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice Salazar, Vanessa Jagger, Brett W. Mongkolsapaya, Juthathip Burgomaster, Katherine E. Dejnirattisai, Wanwisa Winkler, Emma S. Fernandez, Estefania Nelson, Christopher A. Fremont, Daved H. Pierson, Theodore C. Crowe, James E. Screaton, Gavin R. Diamond, Michael S. Cell Rep Article Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although two SPOV strains failed to cause disease in immunocompetent mice, each accumulated in the brain, spleen, eye, testis, and kidney when type I interferon signaling was blocked and unexpectedly caused infection, immune cell infiltration, and swelling in the ankle. In pregnant mice, SPOV replicated in the placenta and fetus but did not cause placental insufficiency or microcephaly. We identified human antibodies from ZIKV or DENV immune subjects that neutralized SPOV infection and protected against lethal challenge. Our experiments describe similarities and differences in clinical syndromes between SPOV and ZIKV and suggest that their serological relatedness has implications for antibody therapeutics and flavivirus vaccine development. 2019-02-05 /pmc/articles/PMC6420780/ /pubmed/30726740 http://dx.doi.org/10.1016/j.celrep.2019.01.052 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Salazar, Vanessa
Jagger, Brett W.
Mongkolsapaya, Juthathip
Burgomaster, Katherine E.
Dejnirattisai, Wanwisa
Winkler, Emma S.
Fernandez, Estefania
Nelson, Christopher A.
Fremont, Daved H.
Pierson, Theodore C.
Crowe, James E.
Screaton, Gavin R.
Diamond, Michael S.
Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title_full Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title_fullStr Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title_full_unstemmed Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title_short Dengue and Zika Virus Cross-Reactive Human Monoclonal Antibodies Protect against Spondweni Virus Infection and Pathogenesis in Mice
title_sort dengue and zika virus cross-reactive human monoclonal antibodies protect against spondweni virus infection and pathogenesis in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420780/
https://www.ncbi.nlm.nih.gov/pubmed/30726740
http://dx.doi.org/10.1016/j.celrep.2019.01.052
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