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Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy with PD98059 to induce apoptosis of human gastric cancer MGC-803 cells
OBJECTIVE: PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase. Substantial preclinical evidence has shown an anti-tumor effect of curcumin on various solid tumors. This study aimed to investigate whether curcumin synergistically acts with PD98059 in exerting anti-gastric...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421392/ https://www.ncbi.nlm.nih.gov/pubmed/30727807 http://dx.doi.org/10.1177/0300060518822213 |
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author | Qiang, Zhanrong Meng, Lingyu Yi, Caixia Yu, Lianying Chen, Wenxia Sha, Weihong |
author_facet | Qiang, Zhanrong Meng, Lingyu Yi, Caixia Yu, Lianying Chen, Wenxia Sha, Weihong |
author_sort | Qiang, Zhanrong |
collection | PubMed |
description | OBJECTIVE: PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase. Substantial preclinical evidence has shown an anti-tumor effect of curcumin on various solid tumors. This study aimed to investigate whether curcumin synergistically acts with PD98059 in exerting anti-gastric cancer effects. METHODS: The cell counting kit-8 assay was used to detect cell proliferation of the human gastric cancer MGC-803 cell line. Flow cytometry was performed to detect apoptosis. Western blotting was used to detect phosphatase and tensin homolog (PTEN) and phosphorylated Akt (p-Akt) expression levels. Quantitative reverse transcription-polymerase chain reaction was used to determine microRNA-21 (miR-21). RESULTS: A dose of 5 to 40 µM curcumin inhibited proliferation of MGC-803 cells in a dose- and time-dependent manner. A high dose of curcumin strongly inhibited p-Akt protein expression. With increasing curcumin levels, PTEN expression increased and miR-21 levels decreased. These results suggest that curcumin negatively modulated the miR-21/PTEN/Akt pathway. Moreover, after pretreatment with PD98059, cell apoptosis induced by curcumin was significantly increased. Additionally, the inhibitory effects of curcumin on the miR-21/PTEN/Akt pathway were significantly enhanced. CONCLUSION: PD98059 combined with curcumin may be a potential strategy for managing gastric cancer. |
format | Online Article Text |
id | pubmed-6421392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-64213922019-03-22 Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy with PD98059 to induce apoptosis of human gastric cancer MGC-803 cells Qiang, Zhanrong Meng, Lingyu Yi, Caixia Yu, Lianying Chen, Wenxia Sha, Weihong J Int Med Res Pre-Clinical Research Reports OBJECTIVE: PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase. Substantial preclinical evidence has shown an anti-tumor effect of curcumin on various solid tumors. This study aimed to investigate whether curcumin synergistically acts with PD98059 in exerting anti-gastric cancer effects. METHODS: The cell counting kit-8 assay was used to detect cell proliferation of the human gastric cancer MGC-803 cell line. Flow cytometry was performed to detect apoptosis. Western blotting was used to detect phosphatase and tensin homolog (PTEN) and phosphorylated Akt (p-Akt) expression levels. Quantitative reverse transcription-polymerase chain reaction was used to determine microRNA-21 (miR-21). RESULTS: A dose of 5 to 40 µM curcumin inhibited proliferation of MGC-803 cells in a dose- and time-dependent manner. A high dose of curcumin strongly inhibited p-Akt protein expression. With increasing curcumin levels, PTEN expression increased and miR-21 levels decreased. These results suggest that curcumin negatively modulated the miR-21/PTEN/Akt pathway. Moreover, after pretreatment with PD98059, cell apoptosis induced by curcumin was significantly increased. Additionally, the inhibitory effects of curcumin on the miR-21/PTEN/Akt pathway were significantly enhanced. CONCLUSION: PD98059 combined with curcumin may be a potential strategy for managing gastric cancer. SAGE Publications 2019-02-06 2019-03 /pmc/articles/PMC6421392/ /pubmed/30727807 http://dx.doi.org/10.1177/0300060518822213 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Reports Qiang, Zhanrong Meng, Lingyu Yi, Caixia Yu, Lianying Chen, Wenxia Sha, Weihong Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy with PD98059 to induce apoptosis of human gastric cancer MGC-803 cells |
title | Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy
with PD98059 to induce apoptosis of human gastric cancer MGC-803
cells |
title_full | Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy
with PD98059 to induce apoptosis of human gastric cancer MGC-803
cells |
title_fullStr | Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy
with PD98059 to induce apoptosis of human gastric cancer MGC-803
cells |
title_full_unstemmed | Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy
with PD98059 to induce apoptosis of human gastric cancer MGC-803
cells |
title_short | Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy
with PD98059 to induce apoptosis of human gastric cancer MGC-803
cells |
title_sort | curcumin regulates the mir-21/pten/akt pathway and acts in synergy
with pd98059 to induce apoptosis of human gastric cancer mgc-803
cells |
topic | Pre-Clinical Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421392/ https://www.ncbi.nlm.nih.gov/pubmed/30727807 http://dx.doi.org/10.1177/0300060518822213 |
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