Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli

Green fluorescent protein (GFP) is amenable to recombinant expression in various kinds of cells and is widely used in life science research. We found that the recombinant expression of GFPuv, a commonly-used mutant of GFP, in E. coli produced two distinct molecular species as judged by in-gel fluore...

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Detalles Bibliográficos
Autores principales: Nakatani, Takahiro, Yasui, Norihisa, Tamura, Issei, Yamashita, Atsuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423240/
https://www.ncbi.nlm.nih.gov/pubmed/30886277
http://dx.doi.org/10.1038/s41598-019-41309-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423240/
https://www.ncbi.nlm.nih.gov/pubmed/30886277
http://dx.doi.org/10.1038/s41598-019-41309-8

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