Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs

Target-specific genome editing using engineered nucleases has become widespread in various fields. Long gene knock-in and single-base substitutions can be performed by homologous recombination (HR), but the efficiency is usually very low. To improve the efficiency of knock-in with single-stranded ol...

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Detalles Bibliográficos
Autores principales: Okamoto, Sachiko, Amaishi, Yasunori, Maki, Izumi, Enoki, Tatsuji, Mineno, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423289/
https://www.ncbi.nlm.nih.gov/pubmed/30886178
http://dx.doi.org/10.1038/s41598-019-41121-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423289/
https://www.ncbi.nlm.nih.gov/pubmed/30886178
http://dx.doi.org/10.1038/s41598-019-41121-4

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