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Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs

Target-specific genome editing using engineered nucleases has become widespread in various fields. Long gene knock-in and single-base substitutions can be performed by homologous recombination (HR), but the efficiency is usually very low. To improve the efficiency of knock-in with single-stranded ol...

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Detalles Bibliográficos
Autores principales:	Okamoto, Sachiko, Amaishi, Yasunori, Maki, Izumi, Enoki, Tatsuji, Mineno, Junichi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423289/ https://www.ncbi.nlm.nih.gov/pubmed/30886178 http://dx.doi.org/10.1038/s41598-019-41121-4

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