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Antigen retrieval by citrate solution improves western blot signal

In the present work, we describe and evaluate an additional step to the standard western blot protocol to increase signal strength after revealing. Weak or absence of signal is a common issue in western blot protocol leading to unexpected results. In our Antigen Retrieval for Western Blot Method (AR...

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Detalles Bibliográficos
Autores principales: Patiño-García, Daniel, Rocha-Pérez, Nadia, Moreno, Ricardo D., Orellana, Renan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423990/
https://www.ncbi.nlm.nih.gov/pubmed/30923683
http://dx.doi.org/10.1016/j.mex.2019.02.030