Design of a generic CRISPR-Cas9 approach using the same sgRNA to perform gene editing at distinct loci

BACKGROUND: The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editing is expanding, some limitations remain such as possible off-target effects an...

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Detalles Bibliográficos
Autores principales: Najah, Soumaya, Saulnier, Corinne, Pernodet, Jean-Luc, Bury-Moné, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425556/
https://www.ncbi.nlm.nih.gov/pubmed/30894153
http://dx.doi.org/10.1186/s12896-019-0509-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425556/
https://www.ncbi.nlm.nih.gov/pubmed/30894153
http://dx.doi.org/10.1186/s12896-019-0509-7

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