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Design of a generic CRISPR-Cas9 approach using the same sgRNA to perform gene editing at distinct loci
BACKGROUND: The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editing is expanding, some limitations remain such as possible off-target effects an...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425556/ https://www.ncbi.nlm.nih.gov/pubmed/30894153 http://dx.doi.org/10.1186/s12896-019-0509-7 |