Cargando…

Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells

BACKGROUND: Integrins mediate cell adhesion, migration, and survival by connecting the intracellular machinery with the surrounding extracellular matrix. Previous studies demonstrated the interaction between α(v)β(3) integrin and VEGF type 2 receptor (VEGFR2) in VEGF-induced angiogenesis. DisBa-01,...

Descripción completa

Detalles Bibliográficos
Autores principales: Danilucci, Taís M., Santos, Patty K., Pachane, Bianca C., Pisani, Graziéle F. D., Lino, Rafael L. B., Casali, Bruna C., Altei, Wanessa F., Selistre-de-Araujo, Heloisa S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425665/
https://www.ncbi.nlm.nih.gov/pubmed/30894182
http://dx.doi.org/10.1186/s12964-019-0339-1
_version_ 1783404882470895616
author Danilucci, Taís M.
Santos, Patty K.
Pachane, Bianca C.
Pisani, Graziéle F. D.
Lino, Rafael L. B.
Casali, Bruna C.
Altei, Wanessa F.
Selistre-de-Araujo, Heloisa S.
author_facet Danilucci, Taís M.
Santos, Patty K.
Pachane, Bianca C.
Pisani, Graziéle F. D.
Lino, Rafael L. B.
Casali, Bruna C.
Altei, Wanessa F.
Selistre-de-Araujo, Heloisa S.
author_sort Danilucci, Taís M.
collection PubMed
description BACKGROUND: Integrins mediate cell adhesion, migration, and survival by connecting the intracellular machinery with the surrounding extracellular matrix. Previous studies demonstrated the interaction between α(v)β(3) integrin and VEGF type 2 receptor (VEGFR2) in VEGF-induced angiogenesis. DisBa-01, a recombinant His-tag fusion, RGD-disintegrin from Bothrops alternatus snake venom, binds to α(v)β(3) integrin with nanomolar affinity blocking cell adhesion to the extracellular matrix. Here we present in vitro evidence of a direct interference of DisBa-01 with α(v)β(3)/VEGFR2 cross-talk and its downstream pathways. METHODS: Human umbilical vein (HUVECs) were cultured in plates coated with fibronectin (FN) or vitronectin (VN) and tested for migration, invasion and proliferation assays in the presence of VEGF, DisBa-01 (1000 nM) or VEGF and DisBa-01 simultaneously. Phosphorylation of α(v)β(3)/VEGFR2 receptors and the activation of intracellular signaling pathways were analyzed by western blotting. Morphological alterations were observed and quantified by fluorescence confocal microscopy. RESULTS: DisBa-01 treatment of endothelial cells inhibited critical steps of VEGF-mediated angiogenesis such as migration, invasion and tubulogenesis. The blockage of α(v)β(3)/VEGFR2 cross-talk by this disintegrin decreases protein expression and phosphorylation of VEGFR2 and β(3) integrin subunit, regulates FAK/SrC/Paxillin downstream signals, and inhibits ERK1/2 and PI3K pathways. These events result in actin re-organization and inhibition of HUVEC migration and adhesion. Labelled-DisBa-01 colocalizes with α(v)β(3) integrin and VEGFR2 in treated cells. CONCLUSIONS: Disintegrin inhibition of α(v)β(3) integrin blocks VEGFR2 signalling, even in the presence of VEGF, which impairs the angiogenic mechanism. These results improve our understanding concerning the mechanisms of pharmacological inhibition of angiogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12964-019-0339-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6425665
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-64256652019-04-01 Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells Danilucci, Taís M. Santos, Patty K. Pachane, Bianca C. Pisani, Graziéle F. D. Lino, Rafael L. B. Casali, Bruna C. Altei, Wanessa F. Selistre-de-Araujo, Heloisa S. Cell Commun Signal Research BACKGROUND: Integrins mediate cell adhesion, migration, and survival by connecting the intracellular machinery with the surrounding extracellular matrix. Previous studies demonstrated the interaction between α(v)β(3) integrin and VEGF type 2 receptor (VEGFR2) in VEGF-induced angiogenesis. DisBa-01, a recombinant His-tag fusion, RGD-disintegrin from Bothrops alternatus snake venom, binds to α(v)β(3) integrin with nanomolar affinity blocking cell adhesion to the extracellular matrix. Here we present in vitro evidence of a direct interference of DisBa-01 with α(v)β(3)/VEGFR2 cross-talk and its downstream pathways. METHODS: Human umbilical vein (HUVECs) were cultured in plates coated with fibronectin (FN) or vitronectin (VN) and tested for migration, invasion and proliferation assays in the presence of VEGF, DisBa-01 (1000 nM) or VEGF and DisBa-01 simultaneously. Phosphorylation of α(v)β(3)/VEGFR2 receptors and the activation of intracellular signaling pathways were analyzed by western blotting. Morphological alterations were observed and quantified by fluorescence confocal microscopy. RESULTS: DisBa-01 treatment of endothelial cells inhibited critical steps of VEGF-mediated angiogenesis such as migration, invasion and tubulogenesis. The blockage of α(v)β(3)/VEGFR2 cross-talk by this disintegrin decreases protein expression and phosphorylation of VEGFR2 and β(3) integrin subunit, regulates FAK/SrC/Paxillin downstream signals, and inhibits ERK1/2 and PI3K pathways. These events result in actin re-organization and inhibition of HUVEC migration and adhesion. Labelled-DisBa-01 colocalizes with α(v)β(3) integrin and VEGFR2 in treated cells. CONCLUSIONS: Disintegrin inhibition of α(v)β(3) integrin blocks VEGFR2 signalling, even in the presence of VEGF, which impairs the angiogenic mechanism. These results improve our understanding concerning the mechanisms of pharmacological inhibition of angiogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12964-019-0339-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-20 /pmc/articles/PMC6425665/ /pubmed/30894182 http://dx.doi.org/10.1186/s12964-019-0339-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Danilucci, Taís M.
Santos, Patty K.
Pachane, Bianca C.
Pisani, Graziéle F. D.
Lino, Rafael L. B.
Casali, Bruna C.
Altei, Wanessa F.
Selistre-de-Araujo, Heloisa S.
Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title_full Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title_fullStr Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title_full_unstemmed Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title_short Recombinant RGD-disintegrin DisBa-01 blocks integrin α(v)β(3) and impairs VEGF signaling in endothelial cells
title_sort recombinant rgd-disintegrin disba-01 blocks integrin α(v)β(3) and impairs vegf signaling in endothelial cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425665/
https://www.ncbi.nlm.nih.gov/pubmed/30894182
http://dx.doi.org/10.1186/s12964-019-0339-1
work_keys_str_mv AT daniluccitaism recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT santospattyk recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT pachanebiancac recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT pisanigrazielefd recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT linorafaellb recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT casalibrunac recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT alteiwanessaf recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells
AT selistredearaujoheloisas recombinantrgddisintegrindisba01blocksintegrinavb3andimpairsvegfsignalinginendothelialcells