Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

BACKGROUND: Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a cell-type specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling. Limited cell nu...

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Detalles Bibliográficos
Autores principales: Loontiens, Siebe, Depestel, Lisa, Vanhauwaert, Suzanne, Dewyn, Givani, Gistelinck, Charlotte, Verboom, Karen, Van Loocke, Wouter, Matthijssens, Filip, Willaert, Andy, Vandesompele, Jo, Speleman, Frank, Durinck, Kaat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425699/
https://www.ncbi.nlm.nih.gov/pubmed/30894119
http://dx.doi.org/10.1186/s12864-019-5608-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425699/
https://www.ncbi.nlm.nih.gov/pubmed/30894119
http://dx.doi.org/10.1186/s12864-019-5608-2

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