Hybrid mass spectrometry methods reveal lot-to-lot differences and delineate the effects of glycosylation on the tertiary structure of Herceptin®

To quantify the measurable variations in the structure of a biopharmaceutical product we systematically evaluate three lots of Herceptin®, two mAb standards and an intact Fc-hinge fragment. Each mAb is examined in three states; glycan intact, truncated (following endoS2 treatment) and fully deglycos...

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Detalles Bibliográficos
Autores principales: Upton, Rosie, Migas, Lukasz G., Pacholarz, Kamila J., Beniston, Richard G., Estdale, Sian, Firth, David, Barran, Perdita E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425993/
https://www.ncbi.nlm.nih.gov/pubmed/30997002
http://dx.doi.org/10.1039/c8sc05029e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425993/
https://www.ncbi.nlm.nih.gov/pubmed/30997002
http://dx.doi.org/10.1039/c8sc05029e

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