Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9

Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an...

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Detalles Bibliográficos
Autores principales: Jeong, You Kyeong, Yu, Jihyeon, Bae, Sangsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426851/
https://www.ncbi.nlm.nih.gov/pubmed/30894632
http://dx.doi.org/10.1038/s41598-019-41356-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426851/
https://www.ncbi.nlm.nih.gov/pubmed/30894632
http://dx.doi.org/10.1038/s41598-019-41356-1

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