A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in Cryptococcus gattii

Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococcus neofo...

Descripción completa

Detalles Bibliográficos
Autores principales: Reis, Flavia C. G., Borges, Beatriz S., Jozefowicz, Luísa J., Sena, Bianca A. G., Garcia, Ane W. A., Medeiros, Lia C., Martins, Sharon T., Honorato, Leandro, Schrank, Augusto, Vainstein, Marilene H., Kmetzsch, Livia, Nimrichter, Leonardo, Alves, Lysangela R., Staats, Charley C., Rodrigues, Marcio L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429041/
https://www.ncbi.nlm.nih.gov/pubmed/30894430
http://dx.doi.org/10.1128/mSphere.00080-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429041/
https://www.ncbi.nlm.nih.gov/pubmed/30894430
http://dx.doi.org/10.1128/mSphere.00080-19

Ejemplares similares