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Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module

The clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9) technology is a versatile and useful tool to perform genome editing in different organisms ranging from bacteria and yeast to plants and mammalian cells. For a couple of years, it was believed that...

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Detalles Bibliográficos
Autores principales: Guzmán-Zapata, Daniel, Sandoval-Vargas, José M., Macedo-Osorio, Karla S., Salgado-Manjarrez, Edgar, Castrejón-Flores, José L., Oliver-Salvador, María del Carmen, Durán-Figueroa, Noé V., Nogué, Fabien, Badillo-Corona, Jesús A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429146/
https://www.ncbi.nlm.nih.gov/pubmed/30871076
http://dx.doi.org/10.3390/ijms20051247