Cargando…

Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons

Neuronal circuits within the prefrontal cortex (PFC) mediate higher cognitive functions and emotional regulation that are disrupted in psychiatric disorders. The PFC undergoes significant maturation during adolescence, a period when cannabis use in humans has been linked to subsequent vulnerability...

Descripción completa

Detalles Bibliográficos
Autores principales: Miller, Michael L., Chadwick, Benjamin, Dickstein, Dara L., Purushothaman, Immanuel, Egervari, Gabor, Rahman, Tanni, Tessereau, Chloe, Hof, Patrick R., Roussos, Panos, Shen, Li, Baxter, Mark G., Hurd, Yasmin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430678/
https://www.ncbi.nlm.nih.gov/pubmed/30283037
http://dx.doi.org/10.1038/s41380-018-0243-x
_version_ 1783405798921076736
author Miller, Michael L.
Chadwick, Benjamin
Dickstein, Dara L.
Purushothaman, Immanuel
Egervari, Gabor
Rahman, Tanni
Tessereau, Chloe
Hof, Patrick R.
Roussos, Panos
Shen, Li
Baxter, Mark G.
Hurd, Yasmin L.
author_facet Miller, Michael L.
Chadwick, Benjamin
Dickstein, Dara L.
Purushothaman, Immanuel
Egervari, Gabor
Rahman, Tanni
Tessereau, Chloe
Hof, Patrick R.
Roussos, Panos
Shen, Li
Baxter, Mark G.
Hurd, Yasmin L.
author_sort Miller, Michael L.
collection PubMed
description Neuronal circuits within the prefrontal cortex (PFC) mediate higher cognitive functions and emotional regulation that are disrupted in psychiatric disorders. The PFC undergoes significant maturation during adolescence, a period when cannabis use in humans has been linked to subsequent vulnerability to psychiatric disorders such as addiction and schizophrenia. Here, we investigated in a rat model the effects of adolescent exposure to Δ(9)-tetrahydrocannabinol (THC), a psychoactive component of cannabis, on the morphological architecture and transcriptional profile of layer III pyramidal neurons—using cell type- and layer-specific high-resolution microscopy, laser capture microdissection and next-generation RNA-sequencing. The results confirmed known normal expansions in basal dendritic arborization and dendritic spine pruning during the transition from late adolescence to early adulthood that were accompanied by differential expression of gene networks associated with neurodevelopment in control animals. In contrast, THC exposure disrupted the normal developmental process by inducing premature pruning of dendritic spines and allostatic atrophy of dendritic arborization in early adulthood. Surprisingly, there was minimal overlap of the developmental transcriptomes between THC- and vehicle-exposed rats. THC altered functional gene networks related to cell morphogenesis, dendritic development, and cytoskeleton organization. Marked developmental network disturbances were evident for epigenetic regulators with enhanced co-expression of chromatin- and dendrite-related genes in THC-treated animals. Dysregulated PFC co-expression networks common to both the THC-treated animals and patients with schizophrenia were enriched for cytoskeletal and neurite development. Overall, adolescent THC exposure altered the morphological and transcriptional trajectory of PFC pyramidal neurons, which could enhance vulnerability to psychiatric disorders.
format Online
Article
Text
id pubmed-6430678
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-64306782019-04-03 Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons Miller, Michael L. Chadwick, Benjamin Dickstein, Dara L. Purushothaman, Immanuel Egervari, Gabor Rahman, Tanni Tessereau, Chloe Hof, Patrick R. Roussos, Panos Shen, Li Baxter, Mark G. Hurd, Yasmin L. Mol Psychiatry Article Neuronal circuits within the prefrontal cortex (PFC) mediate higher cognitive functions and emotional regulation that are disrupted in psychiatric disorders. The PFC undergoes significant maturation during adolescence, a period when cannabis use in humans has been linked to subsequent vulnerability to psychiatric disorders such as addiction and schizophrenia. Here, we investigated in a rat model the effects of adolescent exposure to Δ(9)-tetrahydrocannabinol (THC), a psychoactive component of cannabis, on the morphological architecture and transcriptional profile of layer III pyramidal neurons—using cell type- and layer-specific high-resolution microscopy, laser capture microdissection and next-generation RNA-sequencing. The results confirmed known normal expansions in basal dendritic arborization and dendritic spine pruning during the transition from late adolescence to early adulthood that were accompanied by differential expression of gene networks associated with neurodevelopment in control animals. In contrast, THC exposure disrupted the normal developmental process by inducing premature pruning of dendritic spines and allostatic atrophy of dendritic arborization in early adulthood. Surprisingly, there was minimal overlap of the developmental transcriptomes between THC- and vehicle-exposed rats. THC altered functional gene networks related to cell morphogenesis, dendritic development, and cytoskeleton organization. Marked developmental network disturbances were evident for epigenetic regulators with enhanced co-expression of chromatin- and dendrite-related genes in THC-treated animals. Dysregulated PFC co-expression networks common to both the THC-treated animals and patients with schizophrenia were enriched for cytoskeletal and neurite development. Overall, adolescent THC exposure altered the morphological and transcriptional trajectory of PFC pyramidal neurons, which could enhance vulnerability to psychiatric disorders. Nature Publishing Group UK 2018-10-03 2019 /pmc/articles/PMC6430678/ /pubmed/30283037 http://dx.doi.org/10.1038/s41380-018-0243-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Miller, Michael L.
Chadwick, Benjamin
Dickstein, Dara L.
Purushothaman, Immanuel
Egervari, Gabor
Rahman, Tanni
Tessereau, Chloe
Hof, Patrick R.
Roussos, Panos
Shen, Li
Baxter, Mark G.
Hurd, Yasmin L.
Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title_full Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title_fullStr Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title_full_unstemmed Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title_short Adolescent exposure to Δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
title_sort adolescent exposure to δ(9)-tetrahydrocannabinol alters the transcriptional trajectory and dendritic architecture of prefrontal pyramidal neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430678/
https://www.ncbi.nlm.nih.gov/pubmed/30283037
http://dx.doi.org/10.1038/s41380-018-0243-x
work_keys_str_mv AT millermichaell adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT chadwickbenjamin adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT dicksteindaral adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT purushothamanimmanuel adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT egervarigabor adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT rahmantanni adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT tessereauchloe adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT hofpatrickr adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT roussospanos adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT shenli adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT baxtermarkg adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons
AT hurdyasminl adolescentexposuretod9tetrahydrocannabinolaltersthetranscriptionaltrajectoryanddendriticarchitectureofprefrontalpyramidalneurons