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Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum
BACKGROUND: Corynebacterium glutamicum is an important industrial strain for the production of a diverse range of chemicals. Cpf1 nucleases are highly specific and programmable, with efficiencies comparable to those of Cas9. Although the Francisella novicida (Fn) CRISPR-Cpf1 system has been adapted...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432761/ https://www.ncbi.nlm.nih.gov/pubmed/30909908 http://dx.doi.org/10.1186/s12934-019-1109-x |