Cargando…

Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation

Corn syrup - a commercial product derived from saccharification of corn starch - was used to produce acetone-butanol-ethanol (ABE) by Clostridium spp. Screening of commercial Clostridium spp., substrate inhibition tests and fed-batch experiments were carried out to improve ABE production using corn...

Descripción completa

Detalles Bibliográficos
Autores principales: Niglio, Saverio, Marzocchella, Antonio, Rehmann, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434287/
https://www.ncbi.nlm.nih.gov/pubmed/30963127
http://dx.doi.org/10.1016/j.heliyon.2019.e01401
_version_ 1783406451012665344
author Niglio, Saverio
Marzocchella, Antonio
Rehmann, Lars
author_facet Niglio, Saverio
Marzocchella, Antonio
Rehmann, Lars
author_sort Niglio, Saverio
collection PubMed
description Corn syrup - a commercial product derived from saccharification of corn starch - was used to produce acetone-butanol-ethanol (ABE) by Clostridium spp. Screening of commercial Clostridium spp., substrate inhibition tests and fed-batch experiments were carried out to improve ABE production using corn syrup as only carbon source. The screening tests carried out in batch mode using a production media containing 50 g/L corn syrup revealed that C. saccharobutylicum was the best performer in terms of total solvent concentration (12.46 g/L), yield (0.30 g/g) and productivity (0.19 g/L/h) and it was selected for successive experiments. Concentration of corn syrup higher than 50 g/L resulted in no solvents production. Fed-batch fermentation improved ABE production with respect to batch fermentation: the butanol and solvent concentration increased up to 8.70 and 16.68 g/L, respectively. The study demonstrated the feasibility of producing solvents via ABE fermentation using corn syrup as a model substrate of concentrated sugar mixtures.
format Online
Article
Text
id pubmed-6434287
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-64342872019-04-08 Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation Niglio, Saverio Marzocchella, Antonio Rehmann, Lars Heliyon Article Corn syrup - a commercial product derived from saccharification of corn starch - was used to produce acetone-butanol-ethanol (ABE) by Clostridium spp. Screening of commercial Clostridium spp., substrate inhibition tests and fed-batch experiments were carried out to improve ABE production using corn syrup as only carbon source. The screening tests carried out in batch mode using a production media containing 50 g/L corn syrup revealed that C. saccharobutylicum was the best performer in terms of total solvent concentration (12.46 g/L), yield (0.30 g/g) and productivity (0.19 g/L/h) and it was selected for successive experiments. Concentration of corn syrup higher than 50 g/L resulted in no solvents production. Fed-batch fermentation improved ABE production with respect to batch fermentation: the butanol and solvent concentration increased up to 8.70 and 16.68 g/L, respectively. The study demonstrated the feasibility of producing solvents via ABE fermentation using corn syrup as a model substrate of concentrated sugar mixtures. Elsevier 2019-03-22 /pmc/articles/PMC6434287/ /pubmed/30963127 http://dx.doi.org/10.1016/j.heliyon.2019.e01401 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Niglio, Saverio
Marzocchella, Antonio
Rehmann, Lars
Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title_full Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title_fullStr Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title_full_unstemmed Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title_short Clostridial conversion of corn syrup to Acetone-Butanol-Ethanol (ABE) via batch and fed-batch fermentation
title_sort clostridial conversion of corn syrup to acetone-butanol-ethanol (abe) via batch and fed-batch fermentation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434287/
https://www.ncbi.nlm.nih.gov/pubmed/30963127
http://dx.doi.org/10.1016/j.heliyon.2019.e01401
work_keys_str_mv AT nigliosaverio clostridialconversionofcornsyruptoacetonebutanolethanolabeviabatchandfedbatchfermentation
AT marzocchellaantonio clostridialconversionofcornsyruptoacetonebutanolethanolabeviabatchandfedbatchfermentation
AT rehmannlars clostridialconversionofcornsyruptoacetonebutanolethanolabeviabatchandfedbatchfermentation