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Alveolus-like organoid from isolated tip epithelium of embryonic mouse lung

Embryonic lungs were obtained from embryonic day 13.5 ICR mice. The lung-tip epithelium isolated using dispase treatment was embedded in low-growth factor Matrigel, cultured in DMEM/F12 medium containing 0.1% bovine serum albumin, supplemented with insulin, transferrin, and selenium (ITS), with or w...

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Detalles Bibliográficos
Autores principales: Seiji, Yukihiro, Ito, Takaaki, Nakamura, Yasuko, Nakaishi-Fukuchi, Yuko, Matsuo, Akira, Sato, Naruki, Nogawa, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Japan 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437130/
https://www.ncbi.nlm.nih.gov/pubmed/30635859
http://dx.doi.org/10.1007/s13577-019-00236-6