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Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list

Differential gene expression analysis may discover a set of genes too large to easily investigate, so a means of ranking genes by biological interest level is desired. p values are frequently abused for this purpose. As an alternative, we propose a method of ranking by confidence bounds on the log f...

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Detalles Bibliográficos
Autores principales: Harrison, Paul F., Pattison, Andrew D., Powell, David R., Beilharz, Traude H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437914/
https://www.ncbi.nlm.nih.gov/pubmed/30922379
http://dx.doi.org/10.1186/s13059-019-1674-7
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author Harrison, Paul F.
Pattison, Andrew D.
Powell, David R.
Beilharz, Traude H.
author_facet Harrison, Paul F.
Pattison, Andrew D.
Powell, David R.
Beilharz, Traude H.
author_sort Harrison, Paul F.
collection PubMed
description Differential gene expression analysis may discover a set of genes too large to easily investigate, so a means of ranking genes by biological interest level is desired. p values are frequently abused for this purpose. As an alternative, we propose a method of ranking by confidence bounds on the log fold change, based on the previously developed TREAT test. These confidence bounds provide guaranteed false discovery rate and false coverage-statement rate control. When applied to a breast cancer dataset, the top-ranked genes by Topconfects emphasize markedly different biological processes compared to the top-ranked genes by p value. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1674-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-64379142019-04-08 Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list Harrison, Paul F. Pattison, Andrew D. Powell, David R. Beilharz, Traude H. Genome Biol Method Differential gene expression analysis may discover a set of genes too large to easily investigate, so a means of ranking genes by biological interest level is desired. p values are frequently abused for this purpose. As an alternative, we propose a method of ranking by confidence bounds on the log fold change, based on the previously developed TREAT test. These confidence bounds provide guaranteed false discovery rate and false coverage-statement rate control. When applied to a breast cancer dataset, the top-ranked genes by Topconfects emphasize markedly different biological processes compared to the top-ranked genes by p value. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1674-7) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-28 /pmc/articles/PMC6437914/ /pubmed/30922379 http://dx.doi.org/10.1186/s13059-019-1674-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Method
Harrison, Paul F.
Pattison, Andrew D.
Powell, David R.
Beilharz, Traude H.
Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title_full Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title_fullStr Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title_full_unstemmed Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title_short Topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
title_sort topconfects: a package for confident effect sizes in differential expression analysis provides a more biologically useful ranked gene list
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437914/
https://www.ncbi.nlm.nih.gov/pubmed/30922379
http://dx.doi.org/10.1186/s13059-019-1674-7
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