Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial DNA from samples, but this compromises RNA qu...

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Detalles Bibliográficos
Autores principales: Paska, Csilla, Barta, Imre, Drozdovszky, Orsolya, Antus, Balazs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438495/
https://www.ncbi.nlm.nih.gov/pubmed/30921416
http://dx.doi.org/10.1371/journal.pone.0214609

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438495/
https://www.ncbi.nlm.nih.gov/pubmed/30921416
http://dx.doi.org/10.1371/journal.pone.0214609

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