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Suspending samples over carbon holey films increases heterogeneity of molecular orientations in negative stain electron microscopy
Negative stain electron microscopy (NSEM) is a simple and cost effective method to analyze a wide variety of specimens, especially proteins. In traditional NSEM, the protein sample is applied to and supported by a continuous carbon film. Unfortunately, many proteins stick to the carbon film with a l...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439206/ https://www.ncbi.nlm.nih.gov/pubmed/30976531 http://dx.doi.org/10.1016/j.mex.2019.02.032 |