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FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

BACKGROUND: Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable for secreted proteins requiring proteolytic maturation, such as TGF-β family gro...

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Detalles Bibliográficos
Autores principales: Lemaitre, Régis P., Bogdanova, Aliona, Borgonovo, Barbara, Woodruff, Jeffrey B., Drechsel, David N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441187/
https://www.ncbi.nlm.nih.gov/pubmed/30925874
http://dx.doi.org/10.1186/s12896-019-0512-z