Alpha protons as NMR probes in deuterated proteins

We describe a new labeling method that allows for full protonation at the backbone Hα position, maintaining protein side chains with a high level of deuteration. We refer to the method as alpha proton exchange by transamination (α-PET) since it relies on transaminase activity demonstrated here using...

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Detalles Bibliográficos
Autores principales: Movellan, Kumar Tekwani, Najbauer, Eszter E., Pratihar, Supriya, Salvi, Michele, Giller, Karin, Becker, Stefan, Andreas, Loren B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441447/
https://www.ncbi.nlm.nih.gov/pubmed/30762170
http://dx.doi.org/10.1007/s10858-019-00230-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441447/
https://www.ncbi.nlm.nih.gov/pubmed/30762170
http://dx.doi.org/10.1007/s10858-019-00230-y

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