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Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions
The higher risk of vaso-occlusion events and sudden death for sickle-cell trait (SCT) athletes has been speculatively ascribed to SCT red blood cell (RBC) stiffening during strenuous exercise. However, the microenvironmental changes that could induce the stiffening of SCT RBCs are unknown. To addres...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444709/ https://www.ncbi.nlm.nih.gov/pubmed/31057840 http://dx.doi.org/10.1038/micronano.2016.61 |
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author | Xu, Zhensong Zheng, Yi Wang, Xian Shehata, Nadine Wang, Chen Xie, Shaorong Sun, Yu |
author_facet | Xu, Zhensong Zheng, Yi Wang, Xian Shehata, Nadine Wang, Chen Xie, Shaorong Sun, Yu |
author_sort | Xu, Zhensong |
collection | PubMed |
description | The higher risk of vaso-occlusion events and sudden death for sickle-cell trait (SCT) athletes has been speculatively ascribed to SCT red blood cell (RBC) stiffening during strenuous exercise. However, the microenvironmental changes that could induce the stiffening of SCT RBCs are unknown. To address this question, we measured the mechanical properties of and changes in SCT RBCs under deoxygenated and acidic environments, which are two typical conditions present in the circulation of athletes undertaking strenuous exercise. The results reveal that SCT RBCs are inherently stiffer than RBCs from non-SCT healthy subjects, and a lower pH further stiffens the SCT cells. Furthermore, at both normal and low pH levels, deoxygenation was found to not be the cause of the stiffness of SCT RBCs. This study confirms that the stiffening of SCT RBCs occurs at a low pH and implies that SCT RBC stiffening could be responsible for vaso-occlusion in SCT athletes during strenuous exercise. |
format | Online Article Text |
id | pubmed-6444709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-64447092019-05-03 Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions Xu, Zhensong Zheng, Yi Wang, Xian Shehata, Nadine Wang, Chen Xie, Shaorong Sun, Yu Microsyst Nanoeng Article The higher risk of vaso-occlusion events and sudden death for sickle-cell trait (SCT) athletes has been speculatively ascribed to SCT red blood cell (RBC) stiffening during strenuous exercise. However, the microenvironmental changes that could induce the stiffening of SCT RBCs are unknown. To address this question, we measured the mechanical properties of and changes in SCT RBCs under deoxygenated and acidic environments, which are two typical conditions present in the circulation of athletes undertaking strenuous exercise. The results reveal that SCT RBCs are inherently stiffer than RBCs from non-SCT healthy subjects, and a lower pH further stiffens the SCT cells. Furthermore, at both normal and low pH levels, deoxygenation was found to not be the cause of the stiffness of SCT RBCs. This study confirms that the stiffening of SCT RBCs occurs at a low pH and implies that SCT RBC stiffening could be responsible for vaso-occlusion in SCT athletes during strenuous exercise. Nature Publishing Group 2016-11-07 /pmc/articles/PMC6444709/ /pubmed/31057840 http://dx.doi.org/10.1038/micronano.2016.61 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xu, Zhensong Zheng, Yi Wang, Xian Shehata, Nadine Wang, Chen Xie, Shaorong Sun, Yu Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title | Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title_full | Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title_fullStr | Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title_full_unstemmed | Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title_short | Stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
title_sort | stiffening of sickle cell trait red blood cells under simulated strenuous exercise conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444709/ https://www.ncbi.nlm.nih.gov/pubmed/31057840 http://dx.doi.org/10.1038/micronano.2016.61 |
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