Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema

PURPOSE: To compare and evaluate images of macular cysts with different degrees of reflectivity (from gray to black signal) as observed in B scan spectral domain OCT (SDOCT) and EnFace OCT with decorrelation signal obtained with OCT-angiography (OCTA) in eyes with cystoid macular edema (CME) seconda...

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Autores principales: Farci, Roberta, Sellam, Alexandre, Coscas, Florence, Coscas, Gabriel J., Diaz, Giacomo, Napoli, Pietro Emanuele, Souied, Eric, Galantuomo, Maria Silvana, Fossarello, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446091/
https://www.ncbi.nlm.nih.gov/pubmed/31016197
http://dx.doi.org/10.1155/2019/7835372
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author Farci, Roberta
Sellam, Alexandre
Coscas, Florence
Coscas, Gabriel J.
Diaz, Giacomo
Napoli, Pietro Emanuele
Souied, Eric
Galantuomo, Maria Silvana
Fossarello, Maurizio
author_facet Farci, Roberta
Sellam, Alexandre
Coscas, Florence
Coscas, Gabriel J.
Diaz, Giacomo
Napoli, Pietro Emanuele
Souied, Eric
Galantuomo, Maria Silvana
Fossarello, Maurizio
author_sort Farci, Roberta
collection PubMed
description PURPOSE: To compare and evaluate images of macular cysts with different degrees of reflectivity (from gray to black signal) as observed in B scan spectral domain OCT (SDOCT) and EnFace OCT with decorrelation signal obtained with OCT-angiography (OCTA) in eyes with cystoid macular edema (CME) secondary to diabetic retinopathy (DR) and retinal vein occlusion (RVO). METHODS: Images from 3033 patients affected by CME secondary to diabetes or RVO examined OCTA (Optovue XR Avanti, Optovue, USA) at the University Eye Clinic of Créteil, Hôpital Intercommunal, France, and at the University Eye Clinic of Cagliari, “San Giovanni di Dio” Hospital, Italy, were retrospectively examined. The deep capillary plexus OCTA images and the corresponding EnFace OCT images, both acquired with the same automatic segmentation, had been overlapped to compose RGB color images as red and green channels, respectively, using ImageJ software (National Institutes of Health, Bethesda, MD). Afterward, linear regions of interest were traced on the color images to obtain the profiles of OCTA and EnFace gray values. Number of pixels, mean gray value and standard deviation of the area traced in OCT-A, and EnFace image were analyzed and statistically correlated. Data were exported to Excel to create the plots. RESULTS: 94 patients with DME and 27 patients with RVO showed intraretinal macular cystoid spaces with similar homogeneous, gray-looking content; 73 patients with DME and 113 patients with RVO showed macular cystoid spaces with homogeneous, black-looking content, as observed at SD-OCT, EnFace and OCTA scans. Interestingly, the limits of macular cystoid spaces were clearly detectable with OCTA. The analysis of red and green profiles demonstrated a clearly visible overlap between average OCTA and EnFace signal observed around cystoid spaces that could be attributed to a relationship between the dynamic vascularization and the structural density of the tissue. CONCLUSIONS: This is the first investigation that characterizes and correlates OCTA and EnFace signals on images of macular cystoid spaces in DR and RVO. The low intensity OCTA signals observed inside cystoid spaces raise a relevant question about their nature, as to whether they are due to the presence of corpusculated material pouring out from bloodocular-barrier or they should be considered OCTA artifacts.
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spelling pubmed-64460912019-04-23 Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema Farci, Roberta Sellam, Alexandre Coscas, Florence Coscas, Gabriel J. Diaz, Giacomo Napoli, Pietro Emanuele Souied, Eric Galantuomo, Maria Silvana Fossarello, Maurizio Biomed Res Int Research Article PURPOSE: To compare and evaluate images of macular cysts with different degrees of reflectivity (from gray to black signal) as observed in B scan spectral domain OCT (SDOCT) and EnFace OCT with decorrelation signal obtained with OCT-angiography (OCTA) in eyes with cystoid macular edema (CME) secondary to diabetic retinopathy (DR) and retinal vein occlusion (RVO). METHODS: Images from 3033 patients affected by CME secondary to diabetes or RVO examined OCTA (Optovue XR Avanti, Optovue, USA) at the University Eye Clinic of Créteil, Hôpital Intercommunal, France, and at the University Eye Clinic of Cagliari, “San Giovanni di Dio” Hospital, Italy, were retrospectively examined. The deep capillary plexus OCTA images and the corresponding EnFace OCT images, both acquired with the same automatic segmentation, had been overlapped to compose RGB color images as red and green channels, respectively, using ImageJ software (National Institutes of Health, Bethesda, MD). Afterward, linear regions of interest were traced on the color images to obtain the profiles of OCTA and EnFace gray values. Number of pixels, mean gray value and standard deviation of the area traced in OCT-A, and EnFace image were analyzed and statistically correlated. Data were exported to Excel to create the plots. RESULTS: 94 patients with DME and 27 patients with RVO showed intraretinal macular cystoid spaces with similar homogeneous, gray-looking content; 73 patients with DME and 113 patients with RVO showed macular cystoid spaces with homogeneous, black-looking content, as observed at SD-OCT, EnFace and OCTA scans. Interestingly, the limits of macular cystoid spaces were clearly detectable with OCTA. The analysis of red and green profiles demonstrated a clearly visible overlap between average OCTA and EnFace signal observed around cystoid spaces that could be attributed to a relationship between the dynamic vascularization and the structural density of the tissue. CONCLUSIONS: This is the first investigation that characterizes and correlates OCTA and EnFace signals on images of macular cystoid spaces in DR and RVO. The low intensity OCTA signals observed inside cystoid spaces raise a relevant question about their nature, as to whether they are due to the presence of corpusculated material pouring out from bloodocular-barrier or they should be considered OCTA artifacts. Hindawi 2019-03-20 /pmc/articles/PMC6446091/ /pubmed/31016197 http://dx.doi.org/10.1155/2019/7835372 Text en Copyright © 2019 Roberta Farci et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Farci, Roberta
Sellam, Alexandre
Coscas, Florence
Coscas, Gabriel J.
Diaz, Giacomo
Napoli, Pietro Emanuele
Souied, Eric
Galantuomo, Maria Silvana
Fossarello, Maurizio
Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title_full Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title_fullStr Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title_full_unstemmed Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title_short Multimodal OCT Reflectivity Analysis of the Cystoid Spaces in Cystoid Macular Edema
title_sort multimodal oct reflectivity analysis of the cystoid spaces in cystoid macular edema
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446091/
https://www.ncbi.nlm.nih.gov/pubmed/31016197
http://dx.doi.org/10.1155/2019/7835372
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