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Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines

Ticks harbour and, in many cases transmit to their vertebrate hosts, a wide variety of pathogenic, apathogenic and endosymbiotic microorganisms. Recent molecular analyses have greatly increased the range of bacterial species potentially associated with ticks, but in most cases cannot distinguish bet...

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Autores principales: Palomar, Ana M., Premchand-Branker, Shonnette, Alberdi, Pilar, Belova, Oxana A., Moniuszko-Malinowska, Anna, Kahl, Olaf, Bell-Sakyi, Lesley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446187/
https://www.ncbi.nlm.nih.gov/pubmed/30819609
http://dx.doi.org/10.1016/j.ttbdis.2019.02.008
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author Palomar, Ana M.
Premchand-Branker, Shonnette
Alberdi, Pilar
Belova, Oxana A.
Moniuszko-Malinowska, Anna
Kahl, Olaf
Bell-Sakyi, Lesley
author_facet Palomar, Ana M.
Premchand-Branker, Shonnette
Alberdi, Pilar
Belova, Oxana A.
Moniuszko-Malinowska, Anna
Kahl, Olaf
Bell-Sakyi, Lesley
author_sort Palomar, Ana M.
collection PubMed
description Ticks harbour and, in many cases transmit to their vertebrate hosts, a wide variety of pathogenic, apathogenic and endosymbiotic microorganisms. Recent molecular analyses have greatly increased the range of bacterial species potentially associated with ticks, but in most cases cannot distinguish between surface contaminants, microorganisms present in the remains of the previous blood meal and truly intracellular or tissue-associated bacteria. Here we demonstrate how tick cell lines, primary cell cultures and organ cultures can be used to isolate and propagate bacteria from within embryonic and adult Ixodes ricinus, Dermacentor marginatus and Dermacentor reticulatus ticks originating from different parts of Europe. We isolated and partially characterised four new strains of Spiroplasma from The Netherlands, Spain and Poland, two new strains of Rickettsia raoultii from Russia and Poland, one strain of Rickettsia slovaca from Spain and a species of Mycobacterium from the UK. Comparison with published sequences showed that the Spiroplasma strains were closely related to Spiroplasma ixodetis and the Mycobacterium isolate belonged to the Mycobacterium chelonae complex, while the R. raoultii and R. slovaca strains were similar to previously-validated species.
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spelling pubmed-64461872019-04-15 Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines Palomar, Ana M. Premchand-Branker, Shonnette Alberdi, Pilar Belova, Oxana A. Moniuszko-Malinowska, Anna Kahl, Olaf Bell-Sakyi, Lesley Ticks Tick Borne Dis Article Ticks harbour and, in many cases transmit to their vertebrate hosts, a wide variety of pathogenic, apathogenic and endosymbiotic microorganisms. Recent molecular analyses have greatly increased the range of bacterial species potentially associated with ticks, but in most cases cannot distinguish between surface contaminants, microorganisms present in the remains of the previous blood meal and truly intracellular or tissue-associated bacteria. Here we demonstrate how tick cell lines, primary cell cultures and organ cultures can be used to isolate and propagate bacteria from within embryonic and adult Ixodes ricinus, Dermacentor marginatus and Dermacentor reticulatus ticks originating from different parts of Europe. We isolated and partially characterised four new strains of Spiroplasma from The Netherlands, Spain and Poland, two new strains of Rickettsia raoultii from Russia and Poland, one strain of Rickettsia slovaca from Spain and a species of Mycobacterium from the UK. Comparison with published sequences showed that the Spiroplasma strains were closely related to Spiroplasma ixodetis and the Mycobacterium isolate belonged to the Mycobacterium chelonae complex, while the R. raoultii and R. slovaca strains were similar to previously-validated species. Elsevier 2019-04 /pmc/articles/PMC6446187/ /pubmed/30819609 http://dx.doi.org/10.1016/j.ttbdis.2019.02.008 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palomar, Ana M.
Premchand-Branker, Shonnette
Alberdi, Pilar
Belova, Oxana A.
Moniuszko-Malinowska, Anna
Kahl, Olaf
Bell-Sakyi, Lesley
Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title_full Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title_fullStr Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title_full_unstemmed Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title_short Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines
title_sort isolation of known and potentially pathogenic tick-borne microorganisms from european ixodid ticks using tick cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446187/
https://www.ncbi.nlm.nih.gov/pubmed/30819609
http://dx.doi.org/10.1016/j.ttbdis.2019.02.008
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