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Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform

As an RNA-guided nuclease, CRISPR-Cas9 offers facile and promising solutions to mediate genome modification with respect to versatility and high precision. However, spatiotemporal manipulation of CRISPR-Cas9 delivery remains a daunting challenge for robust effectuation of gene editing both in vitro...

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Autores principales: Pan, Yongchun, Yang, Jingjing, Luan, Xiaowei, Liu, Xinli, Li, Xueqing, Yang, Jian, Huang, Ting, Sun, Lu, Wang, Yuzhen, Lin, Youhui, Song, Yujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447385/
https://www.ncbi.nlm.nih.gov/pubmed/30949579
http://dx.doi.org/10.1126/sciadv.aav7199
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author Pan, Yongchun
Yang, Jingjing
Luan, Xiaowei
Liu, Xinli
Li, Xueqing
Yang, Jian
Huang, Ting
Sun, Lu
Wang, Yuzhen
Lin, Youhui
Song, Yujun
author_facet Pan, Yongchun
Yang, Jingjing
Luan, Xiaowei
Liu, Xinli
Li, Xueqing
Yang, Jian
Huang, Ting
Sun, Lu
Wang, Yuzhen
Lin, Youhui
Song, Yujun
author_sort Pan, Yongchun
collection PubMed
description As an RNA-guided nuclease, CRISPR-Cas9 offers facile and promising solutions to mediate genome modification with respect to versatility and high precision. However, spatiotemporal manipulation of CRISPR-Cas9 delivery remains a daunting challenge for robust effectuation of gene editing both in vitro and in vivo. Here, we designed a near-infrared (NIR) light–responsive nanocarrier of CRISPR-Cas9 for cancer therapeutics based on upconversion nanoparticles (UCNPs). The UCNPs served as “nanotransducers” that can convert NIR light (980 nm) into local ultraviolet light for the cleavage of photosensitive molecules, thereby resulting in on-demand release of CRISPR-Cas9. In addition, by preparing a single guide RNA targeting a tumor gene (polo-like kinase-1), our strategies have successfully inhibited the proliferation of tumor cell via NIR light–activated gene editing both in vitro and in vivo. Overall, this exogenously controlled method presents enormous potential for targeted gene editing in deep tissues and treatment of a myriad of diseases.
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spelling pubmed-64473852019-04-04 Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform Pan, Yongchun Yang, Jingjing Luan, Xiaowei Liu, Xinli Li, Xueqing Yang, Jian Huang, Ting Sun, Lu Wang, Yuzhen Lin, Youhui Song, Yujun Sci Adv Research Articles As an RNA-guided nuclease, CRISPR-Cas9 offers facile and promising solutions to mediate genome modification with respect to versatility and high precision. However, spatiotemporal manipulation of CRISPR-Cas9 delivery remains a daunting challenge for robust effectuation of gene editing both in vitro and in vivo. Here, we designed a near-infrared (NIR) light–responsive nanocarrier of CRISPR-Cas9 for cancer therapeutics based on upconversion nanoparticles (UCNPs). The UCNPs served as “nanotransducers” that can convert NIR light (980 nm) into local ultraviolet light for the cleavage of photosensitive molecules, thereby resulting in on-demand release of CRISPR-Cas9. In addition, by preparing a single guide RNA targeting a tumor gene (polo-like kinase-1), our strategies have successfully inhibited the proliferation of tumor cell via NIR light–activated gene editing both in vitro and in vivo. Overall, this exogenously controlled method presents enormous potential for targeted gene editing in deep tissues and treatment of a myriad of diseases. American Association for the Advancement of Science 2019-04-03 /pmc/articles/PMC6447385/ /pubmed/30949579 http://dx.doi.org/10.1126/sciadv.aav7199 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Pan, Yongchun
Yang, Jingjing
Luan, Xiaowei
Liu, Xinli
Li, Xueqing
Yang, Jian
Huang, Ting
Sun, Lu
Wang, Yuzhen
Lin, Youhui
Song, Yujun
Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title_full Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title_fullStr Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title_full_unstemmed Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title_short Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
title_sort near-infrared upconversion–activated crispr-cas9 system: a remote-controlled gene editing platform
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447385/
https://www.ncbi.nlm.nih.gov/pubmed/30949579
http://dx.doi.org/10.1126/sciadv.aav7199
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