Novel parameter describing restriction endonucleases: Secondary-Cognate-Specificity and chemical stimulation of TsoI leading to substrate specificity change

Over 470 prototype Type II restriction endonucleases (REases) are currently known. Most recognise specific DNA sequences 4–8 bp long, with very few exceptions cleaving DNA more frequently. TsoI is a thermostable Type IIC enzyme that recognises the DNA sequence TARCCA (R = A or G) and cleaves downstr...

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Detalles Bibliográficos
Autores principales: Zebrowska, Joanna, Jezewska-Frackowiak, Joanna, Wieczerzak, Ewa, Kasprzykowski, Franciszek, Zylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449304/
https://www.ncbi.nlm.nih.gov/pubmed/30879089
http://dx.doi.org/10.1007/s00253-019-09731-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449304/
https://www.ncbi.nlm.nih.gov/pubmed/30879089
http://dx.doi.org/10.1007/s00253-019-09731-0

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