Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming

Primed epiblast stem cells (EpiSCs) can be reverted to a pluripotent embryonic stem cell (ESC)-like state by expression of single reprogramming factor. We used CRISPR activation to perform a genome-scale, reprogramming screen in EpiSCs and identified 142 candidate genes. Our screen validated a total...

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Detalles Bibliográficos
Autores principales: Yang, Jian, Rajan, Sandeep S., Friedrich, Mathias J., Lan, Guocheng, Zou, Xiangang, Ponstingl, Hannes, Garyfallos, Dimitrios A., Liu, Pentao, Bradley, Allan, Metzakopian, Emmanouil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450436/
https://www.ncbi.nlm.nih.gov/pubmed/30905739
http://dx.doi.org/10.1016/j.stemcr.2019.02.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450436/
https://www.ncbi.nlm.nih.gov/pubmed/30905739
http://dx.doi.org/10.1016/j.stemcr.2019.02.010

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