SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping

Mutagenesis libraries are essential for combinatorial protein engineering. Despite improvements in gene synthesis and directed mutagenesis, current methodologies still have limitations regarding the synthesis of complete antibody single-chain variable fragment (scFv) genes and simultaneous diversifi...

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Detalles Bibliográficos
Autores principales: Hu, Francis Jingxin, Lundqvist, Magnus, Uhlén, Mathias, Rockberg, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451119/
https://www.ncbi.nlm.nih.gov/pubmed/30715449
http://dx.doi.org/10.1093/nar/gkz050

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451119/
https://www.ncbi.nlm.nih.gov/pubmed/30715449
http://dx.doi.org/10.1093/nar/gkz050

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