Defining the RNA interactome by total RNA‐associated protein purification

The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)‐associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA‐associated protein purification (TRAPP) based on 254 nm UV crosslinking and...

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Detalles Bibliográficos
Autores principales: Shchepachev, Vadim, Bresson, Stefan, Spanos, Christos, Petfalski, Elisabeth, Fischer, Lutz, Rappsilber, Juri, Tollervey, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452921/
https://www.ncbi.nlm.nih.gov/pubmed/30962360
http://dx.doi.org/10.15252/msb.20188689

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452921/
https://www.ncbi.nlm.nih.gov/pubmed/30962360
http://dx.doi.org/10.15252/msb.20188689

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