Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it is challenging to profile Cas9 activity in the endogenous cellular environment. Here we leverage our TRIP technology to integrate ~ 1k barcoded repor...

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Detalles Bibliográficos
Autores principales: Gisler, Santiago, Gonçalves, Joana P., Akhtar, Waseem, de Jong, Johann, Pindyurin, Alexey V., Wessels, Lodewyk F. A., van Lohuizen, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453899/
https://www.ncbi.nlm.nih.gov/pubmed/30962441
http://dx.doi.org/10.1038/s41467-019-09551-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453899/
https://www.ncbi.nlm.nih.gov/pubmed/30962441
http://dx.doi.org/10.1038/s41467-019-09551-w

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