An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

As the applications of CRISPR-Cas9 technology diversify and spread beyond the laboratory to diagnostic and therapeutic use, the demands of gRNA synthesis have increased and access to tailored gRNAs is now restrictive. Enzymatic routes are time-consuming, difficult to scale-up and suffer from polymer...

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Detalles Bibliográficos
Autores principales: Taemaitree, Lapatrada, Shivalingam, Arun, El-Sagheer, Afaf H., Brown, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453947/
https://www.ncbi.nlm.nih.gov/pubmed/30962447
http://dx.doi.org/10.1038/s41467-019-09600-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453947/
https://www.ncbi.nlm.nih.gov/pubmed/30962447
http://dx.doi.org/10.1038/s41467-019-09600-4

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