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Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays

BACKGROUND: The reduction of tetrazolium salts by NAD(P)H to formazan product has been widely used to determine the metabolic activity of cells, and as an indicator of cell viability. However, the application of a WST-8 based assay for the quantitative measurement of dehydrogenase enzyme activity ha...

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Detalles Bibliográficos
Autores principales: Chamchoy, Kamonwan, Pakotiprapha, Danaya, Pumirat, Pornpan, Leartsakulpanich, Ubolsree, Boonyuen, Usa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454709/
https://www.ncbi.nlm.nih.gov/pubmed/30961528
http://dx.doi.org/10.1186/s12858-019-0108-1