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Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
BACKGROUND: The reduction of tetrazolium salts by NAD(P)H to formazan product has been widely used to determine the metabolic activity of cells, and as an indicator of cell viability. However, the application of a WST-8 based assay for the quantitative measurement of dehydrogenase enzyme activity ha...
Autores principales: | Chamchoy, Kamonwan, Pakotiprapha, Danaya, Pumirat, Pornpan, Leartsakulpanich, Ubolsree, Boonyuen, Usa |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454709/ https://www.ncbi.nlm.nih.gov/pubmed/30961528 http://dx.doi.org/10.1186/s12858-019-0108-1 |
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