Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production

Optimizing microbial hosts for the large-scale production of valuable metabolites often requires multiple mutations and modifications to the host’s genome. We describe a three-round screen for increased L-DOPA production in S. cerevisiae using FACS enrichment of an enzyme-coupled biosensor for L-DOP...

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Detalles Bibliográficos
Autores principales: Savitskaya, Judy, Protzko, Ryan J., Li, Francesca-Zhoufan, Arkin, Adam P., Dueber, John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456618/
https://www.ncbi.nlm.nih.gov/pubmed/30967567
http://dx.doi.org/10.1038/s41598-019-41759-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456618/
https://www.ncbi.nlm.nih.gov/pubmed/30967567
http://dx.doi.org/10.1038/s41598-019-41759-0

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