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Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production
Optimizing microbial hosts for the large-scale production of valuable metabolites often requires multiple mutations and modifications to the host’s genome. We describe a three-round screen for increased L-DOPA production in S. cerevisiae using FACS enrichment of an enzyme-coupled biosensor for L-DOP...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456618/ https://www.ncbi.nlm.nih.gov/pubmed/30967567 http://dx.doi.org/10.1038/s41598-019-41759-0 |