Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species

Talaromyces flavus is a soilborne fungus that can contaminate fruits. It constitutes serious influence on heat-processed food spoilage, as T. flavus belongs to the heat-resistant fungi group, which are able to survive the pasteurization process. Moreover T. flavus has been reported to be capable of...

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Autores principales: Panek, Jacek, Frąc, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458134/
https://www.ncbi.nlm.nih.gov/pubmed/30971732
http://dx.doi.org/10.1038/s41598-019-42275-x
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author Panek, Jacek
Frąc, Magdalena
author_facet Panek, Jacek
Frąc, Magdalena
author_sort Panek, Jacek
collection PubMed
description Talaromyces flavus is a soilborne fungus that can contaminate fruits. It constitutes serious influence on heat-processed food spoilage, as T. flavus belongs to the heat-resistant fungi group, which are able to survive the pasteurization process. Moreover T. flavus has been reported to be capable of mycotoxigenicity, therefore they have a serious threat to human health. To maintain the safety of food production, sensitive method for T. flavus detection was developed. The loop mediated amplification, abbreviated LAMP, reactions were designed as specific for detection of DNA replication licensing factor gene of T. flavus. The specificity of assay was confirmed by use of 5 T. flavus strains and 35 other fungal isolates. The achieved limit of detection was 1fg of T. flavus genomic DNA and 64 ascospores in 1 g of strawberry fruits or soil samples.
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spelling pubmed-64581342019-04-15 Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species Panek, Jacek Frąc, Magdalena Sci Rep Article Talaromyces flavus is a soilborne fungus that can contaminate fruits. It constitutes serious influence on heat-processed food spoilage, as T. flavus belongs to the heat-resistant fungi group, which are able to survive the pasteurization process. Moreover T. flavus has been reported to be capable of mycotoxigenicity, therefore they have a serious threat to human health. To maintain the safety of food production, sensitive method for T. flavus detection was developed. The loop mediated amplification, abbreviated LAMP, reactions were designed as specific for detection of DNA replication licensing factor gene of T. flavus. The specificity of assay was confirmed by use of 5 T. flavus strains and 35 other fungal isolates. The achieved limit of detection was 1fg of T. flavus genomic DNA and 64 ascospores in 1 g of strawberry fruits or soil samples. Nature Publishing Group UK 2019-04-10 /pmc/articles/PMC6458134/ /pubmed/30971732 http://dx.doi.org/10.1038/s41598-019-42275-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Panek, Jacek
Frąc, Magdalena
Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title_full Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title_fullStr Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title_full_unstemmed Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title_short Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
title_sort loop-mediated isothermal amplification (lamp) approach for detection of heat-resistant talaromyces flavus species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458134/
https://www.ncbi.nlm.nih.gov/pubmed/30971732
http://dx.doi.org/10.1038/s41598-019-42275-x
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