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An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS

BACKGROUND: Two-dimensional electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are widely used in plant proteomics research. However, these two techniques cannot be simultaneously satisfied by traditional protein extraction methods when investigate cotton leaf prote...

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Detalles Bibliográficos
Autores principales: Jin, Xiang, Zhu, Liping, Tao, Chengcheng, Xie, Quanliang, Xu, Xinyang, Chang, Lili, Tan, Yanhua, Ding, Guohua, Li, Hongbin, Wang, Xuchu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458646/
https://www.ncbi.nlm.nih.gov/pubmed/30975097
http://dx.doi.org/10.1186/s12864-019-5658-5