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An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS
BACKGROUND: Two-dimensional electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are widely used in plant proteomics research. However, these two techniques cannot be simultaneously satisfied by traditional protein extraction methods when investigate cotton leaf prote...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458646/ https://www.ncbi.nlm.nih.gov/pubmed/30975097 http://dx.doi.org/10.1186/s12864-019-5658-5 |