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An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS

BACKGROUND: Two-dimensional electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are widely used in plant proteomics research. However, these two techniques cannot be simultaneously satisfied by traditional protein extraction methods when investigate cotton leaf prote...

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Detalles Bibliográficos
Autores principales:	Jin, Xiang, Zhu, Liping, Tao, Chengcheng, Xie, Quanliang, Xu, Xinyang, Chang, Lili, Tan, Yanhua, Ding, Guohua, Li, Hongbin, Wang, Xuchu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458646/ https://www.ncbi.nlm.nih.gov/pubmed/30975097 http://dx.doi.org/10.1186/s12864-019-5658-5

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