Quantitative and qualitative evaluation of the impact of the G2 enhancer, bead sizes and lysing tubes on the bacterial community composition during DNA extraction from recalcitrant soil core samples based on community sequencing and qPCR

Soil DNA extraction encounters numerous challenges that can affect both yield and purity of the recovered DNA. Clay particles lead to reduced DNA extraction efficiency, and PCR inhibitors from the soil matrix can negatively affect downstream analyses when applying DNA sequencing. Further, these effe...

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Detalles Bibliográficos
Autores principales: Gobbi, Alex, Santini, Rui G., Filippi, Elisa, Ellegaard-Jensen, Lea, Jacobsen, Carsten S., Hansen, Lars H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459482/
https://www.ncbi.nlm.nih.gov/pubmed/30973938
http://dx.doi.org/10.1371/journal.pone.0200979

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459482/
https://www.ncbi.nlm.nih.gov/pubmed/30973938
http://dx.doi.org/10.1371/journal.pone.0200979

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