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TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn
Ten-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-c...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461695/ https://www.ncbi.nlm.nih.gov/pubmed/30979904 http://dx.doi.org/10.1038/s41467-019-09665-1 |
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author | Montalbán-Loro, Raquel Lozano-Ureña, Anna Ito, Mitsuteru Krueger, Christel Reik, Wolf Ferguson-Smith, Anne C. Ferrón, Sacri R. |
author_facet | Montalbán-Loro, Raquel Lozano-Ureña, Anna Ito, Mitsuteru Krueger, Christel Reik, Wolf Ferguson-Smith, Anne C. Ferrón, Sacri R. |
author_sort | Montalbán-Loro, Raquel |
collection | PubMed |
description | Ten-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-catalytic action of TET3 is essentially required for the maintenance of the neural stem cell (NSC) pool in the adult subventricular zone (SVZ) niche by preventing premature differentiation of NSCs into non-neurogenic astrocytes. This occurs through direct binding of TET3 to the paternal transcribed allele of the imprinted gene Small nuclear ribonucleoprotein-associated polypeptide N (Snrpn), contributing to transcriptional repression of the gene. The study also identifies BMP2 as an effector of the astrocytic terminal differentiation mediated by SNRPN. Our work describes a novel mechanism of control of an imprinted gene in the regulation of adult neurogenesis through an unconventional role of TET3. |
format | Online Article Text |
id | pubmed-6461695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64616952019-04-15 TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn Montalbán-Loro, Raquel Lozano-Ureña, Anna Ito, Mitsuteru Krueger, Christel Reik, Wolf Ferguson-Smith, Anne C. Ferrón, Sacri R. Nat Commun Article Ten-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-catalytic action of TET3 is essentially required for the maintenance of the neural stem cell (NSC) pool in the adult subventricular zone (SVZ) niche by preventing premature differentiation of NSCs into non-neurogenic astrocytes. This occurs through direct binding of TET3 to the paternal transcribed allele of the imprinted gene Small nuclear ribonucleoprotein-associated polypeptide N (Snrpn), contributing to transcriptional repression of the gene. The study also identifies BMP2 as an effector of the astrocytic terminal differentiation mediated by SNRPN. Our work describes a novel mechanism of control of an imprinted gene in the regulation of adult neurogenesis through an unconventional role of TET3. Nature Publishing Group UK 2019-04-12 /pmc/articles/PMC6461695/ /pubmed/30979904 http://dx.doi.org/10.1038/s41467-019-09665-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Montalbán-Loro, Raquel Lozano-Ureña, Anna Ito, Mitsuteru Krueger, Christel Reik, Wolf Ferguson-Smith, Anne C. Ferrón, Sacri R. TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title | TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title_full | TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title_fullStr | TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title_full_unstemmed | TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title_short | TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn |
title_sort | tet3 prevents terminal differentiation of adult nscs by a non-catalytic action at snrpn |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461695/ https://www.ncbi.nlm.nih.gov/pubmed/30979904 http://dx.doi.org/10.1038/s41467-019-09665-1 |
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