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An efficient and robust laboratory workflow and tetrapod database for larger scale environmental DNA studies

BACKGROUND: The use of environmental DNA for species detection via metabarcoding is growing rapidly. We present a co-designed lab workflow and bioinformatic pipeline to mitigate the 2 most important risks of environmental DNA use: sample contamination and taxonomic misassignment. These risks arise f...

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Detalles Bibliográficos
Autores principales: Axtner, Jan, Crampton-Platt, Alex, Hörig, Lisa A, Mohamed, Azlan, Xu, Charles C Y, Yu, Douglas W, Wilting, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461710/
https://www.ncbi.nlm.nih.gov/pubmed/30997489
http://dx.doi.org/10.1093/gigascience/giz029