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Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses

Microbiological testing is an important quality management tool in the food industry. In this study, the hygiene status of beef carcasses sampled in eight Brazilian slaughterhouses was assessed by enumeration of different hygiene indicator microorganisms, and a model to establish potential associati...

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Autores principales: Camargo, Anderson Carlos, Cossi, Marcus Vinícius Coutinho, da Silva, Wladimir Padilha, Bersot, Luciano dos Santos, Landgraf, Mariza, Baranyi, József, Franco, Bernadette Dora Gombossy de Melo, Luís Augusto, Nero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462900/
https://www.ncbi.nlm.nih.gov/pubmed/30893864
http://dx.doi.org/10.3390/microorganisms7030086
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author Camargo, Anderson Carlos
Cossi, Marcus Vinícius Coutinho
da Silva, Wladimir Padilha
Bersot, Luciano dos Santos
Landgraf, Mariza
Baranyi, József
Franco, Bernadette Dora Gombossy de Melo
Luís Augusto, Nero
author_facet Camargo, Anderson Carlos
Cossi, Marcus Vinícius Coutinho
da Silva, Wladimir Padilha
Bersot, Luciano dos Santos
Landgraf, Mariza
Baranyi, József
Franco, Bernadette Dora Gombossy de Melo
Luís Augusto, Nero
author_sort Camargo, Anderson Carlos
collection PubMed
description Microbiological testing is an important quality management tool in the food industry. In this study, the hygiene status of beef carcasses sampled in eight Brazilian slaughterhouses was assessed by enumeration of different hygiene indicator microorganisms, and a model to establish potential associations among these counts was proposed. The carcasses (n = 464) were surface sampled at four slaughtering steps (step 1: Hide after bleeding; step 2: Carcass after hide removal; step 3: Carcass after evisceration; step 4: Carcass after end washing) and subjected to a counting of mesophilic aerobes (MA), Enterobacteriaceae (EB), total coliforms (TC), and Escherichia coli (EC) using Petrifilm™ plates. Among the sampled beef carcasses (step 4), 32 (6.9%) and 71 (15.3%) presented counts above the microbiological criteria established by (EC) No. 1441/2007 for MA and EB, respectively. Thus, indicating that improvements in slaughter hygiene and a review of process controls are demanded in some of the studied slaughterhouses. The log count differences of EC, TC, and EB from MA were considered as response variables as a function of the slaughtering steps. Differential log counts changed consistently with the steps. The measurements, including the patterns in their inherently random variability, were fairly predictable from steps 1 and 4. The results indicated that differential log counts for TC and EC are not relevant, as their concentrations and random pattern can be inferred from counts of MA and EB. The proposed model can be used as a valuable tool for the design and adoption of feasible quality control programs in beef industries. The adoption of such a tool should have a positive contribution on consumers’ health and enhance product quality.
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spelling pubmed-64629002019-04-22 Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses Camargo, Anderson Carlos Cossi, Marcus Vinícius Coutinho da Silva, Wladimir Padilha Bersot, Luciano dos Santos Landgraf, Mariza Baranyi, József Franco, Bernadette Dora Gombossy de Melo Luís Augusto, Nero Microorganisms Article Microbiological testing is an important quality management tool in the food industry. In this study, the hygiene status of beef carcasses sampled in eight Brazilian slaughterhouses was assessed by enumeration of different hygiene indicator microorganisms, and a model to establish potential associations among these counts was proposed. The carcasses (n = 464) were surface sampled at four slaughtering steps (step 1: Hide after bleeding; step 2: Carcass after hide removal; step 3: Carcass after evisceration; step 4: Carcass after end washing) and subjected to a counting of mesophilic aerobes (MA), Enterobacteriaceae (EB), total coliforms (TC), and Escherichia coli (EC) using Petrifilm™ plates. Among the sampled beef carcasses (step 4), 32 (6.9%) and 71 (15.3%) presented counts above the microbiological criteria established by (EC) No. 1441/2007 for MA and EB, respectively. Thus, indicating that improvements in slaughter hygiene and a review of process controls are demanded in some of the studied slaughterhouses. The log count differences of EC, TC, and EB from MA were considered as response variables as a function of the slaughtering steps. Differential log counts changed consistently with the steps. The measurements, including the patterns in their inherently random variability, were fairly predictable from steps 1 and 4. The results indicated that differential log counts for TC and EC are not relevant, as their concentrations and random pattern can be inferred from counts of MA and EB. The proposed model can be used as a valuable tool for the design and adoption of feasible quality control programs in beef industries. The adoption of such a tool should have a positive contribution on consumers’ health and enhance product quality. MDPI 2019-03-19 /pmc/articles/PMC6462900/ /pubmed/30893864 http://dx.doi.org/10.3390/microorganisms7030086 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camargo, Anderson Carlos
Cossi, Marcus Vinícius Coutinho
da Silva, Wladimir Padilha
Bersot, Luciano dos Santos
Landgraf, Mariza
Baranyi, József
Franco, Bernadette Dora Gombossy de Melo
Luís Augusto, Nero
Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title_full Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title_fullStr Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title_full_unstemmed Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title_short Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses
title_sort microbiological testing for the proper assessment of the hygiene status of beef carcasses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462900/
https://www.ncbi.nlm.nih.gov/pubmed/30893864
http://dx.doi.org/10.3390/microorganisms7030086
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