Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Peptide tags are a key resource, introducing minimal change while enabling a consistent process to purify diverse proteins. However, peptide tags often provide minimal benefit post-purification. We previously designed SpyTag, forming an irreversible bond with its protein partner SpyCatcher. SpyTag p...

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Detalles Bibliográficos
Autores principales: Khairil Anuar, Irsyad N. A., Banerjee, Anusuya, Keeble, Anthony H., Carella, Alberto, Nikov, Georgi I., Howarth, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465384/
https://www.ncbi.nlm.nih.gov/pubmed/30988307
http://dx.doi.org/10.1038/s41467-019-09678-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465384/
https://www.ncbi.nlm.nih.gov/pubmed/30988307
http://dx.doi.org/10.1038/s41467-019-09678-w

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